PulmoSmart on Tissue Biopsy
The standard 11-gene NSCLC panel on FFPE tissue — analysing DNA and RNA together from a single sample. Guideline-complete coverage of every actionable driver, delivered in 5–7 days on standard PCR your lab already runs.
What's covered.
99% of DNA mutations and >97% of gene fusions listed in NCCN guidelines (COSMIC v100) — 11 genes, 114 variants, all in a single test.
- EGFR — exon 18/19/20/21 (T790M, C797S, L858R)
- ALK — EML4-ALK and partner fusions
- ROS1 — all clinically relevant fusion partners
- BRAF — V600E
- MET — exon 14 skipping
- KRAS — G12/G13/Q61 hotspots (incl. G12C)
- RET — KIF5B-RET, CCDC6-RET
- ERBB2 (HER2) — exon 20 insertions
- NTRK1/2/3 — all fusion partners
- PIK3CA — hotspot mutations
- PD-L1 — companion testing recommended
Specifications.
- Platform
- QuantStudio™ 5 real-time PCR
- Indication
- Stage IB–IV NSCLC
- Sample
- FFPE tissue block or unstained slides · ≥10% tumor
- Input
- 5 ng DNA / 1.5 ng RNA
- Coverage
- 11 genes · 114 variants · DNA + RNA together
- Turnaround
- 5–7 days from receipt
- LoD₉₅
- <3% VAF (DNA) · <100 copies (fusions)
- Specificity
- 100% · tissue & blood
- Failure rate
- ~1% (vs ~25% NGS norm)
- Report
- Clinician-ready, actionable variants with confidence calls
Built for the samples Indian oncologists actually work with.
DNA + RNA together
One tissue sample, one workflow — no split into two labs or two runs. Every actionable driver, whether it's a mutation or a fusion.
Runs on QuantStudio™ 5
No new capital equipment. If your lab already runs qPCR, it can run PulmoSmart. Setup and training in 3 days.
Built for hard samples
Works with low tumour content and low DNA/RNA input — where standard NGS drops out.
When to choose PulmoSmart.
Adequate tissue on the block, oncologist has 5–7 days to plan the therapy line. The workhorse — most patients, most days. Need it in 2 days? PulmoSmart Prime. Tissue exhausted? LiquiSmart Lung.
Order PulmoSmart Compare all 3 tests