Lung Cancer · Liquid Biopsy · Cancer Resistance Test

LiquiSmart Lung

Ultra-sensitive ctDNA testing for non-small cell lung cancer — from a tube of blood. Rescue when tissue is QNS. Then repeat, to catch treatment resistance as it emerges — often before imaging shows progression. All 11 guideline genes, 2-day turnaround, on standard PCR your lab already runs.

Guideline-complete coverage

11 genes. 114 variants. One test.

Comprehensive coverage of NSCLC markers — 99% of DNA mutations and >97% of gene fusions listed in NCCN guidelines (COSMIC v100). From a single 10 mL blood draw.

11therapy-defining genes
114variants covered
77DNA variants
37RNA fusions
100%specificity
EGFRDNAExon 18/19/20/21 — incl. T790M, C797S, L858R
KRASDNAG12/G13/Q61 hotspots — incl. G12C
BRAFDNAV600E exon 15
ERBB2DNAExon 17 V659E · Exon 20 insertions
PIK3CADNAHotspot mutations
ALKRNA fusionEML4-ALK and partner variants · G1202R resistance
ROS1RNA fusionAll clinically relevant fusion partners
RETRNA fusionKIF5B-RET and CCDC6-RET variants
METRNAExon 14 skipping · amplification bypass
NTRK1/2/3RNA fusionAll NTRK fusion partners
PD-L1proteinCompanion testing recommended
Cancer Resistance Test

Resistance is invisible until it's already advanced.
LiquiSmart makes it visible.

Cancer evolves under therapy. Rebiopsy is invasive and often not feasible. Imaging lags biology by months. A serial ctDNA test turns a single snapshot into a molecular timeline — quantitatively (how much) and qualitatively (why).

On-target resistance

The drug target itself changes, reducing binding.

  • EGFR T790M — classic after early-generation EGFR TKIs
  • EGFR C797S — key mechanism after osimertinib
  • ALK G1202R — kinase-domain mutation on ALK inhibitors

Bypass signalling

The cancer activates another route around the block.

  • MET amplification — a common osimertinib bypass
  • HER2 amplification — alternative growth signalling
  • KRAS / BRAF / PIK3CA — downstream pathway activation

Interpret in context. A negative plasma result does not rule out resistance — some tumours shed little ctDNA, and histological transformation still needs tissue. Molecular findings are integrated with imaging, pathology and clinical assessment. Refs: ESMO NSCLC guideline (Hendriks 2023); Pascual 2022; Thress 2015; Mok 2017; Dagogo-Jack 2020.

Analytical performance

Sensitivity that matches or beats NGS. 100% specificity.

Median panel-wide sensitivity (LoD95). Performance holds at low input, where most NGS workflows fail.

Blood (plasma)10 mL PAXgene®
AssayDNA SNV/indelRNA fusionsMET ex14
LoD₉₅0.3% VAF<6 copies<100 copies
Specificity100%100%100%
Also runs on tissue (FFPE)Rescue when biopsy is QNS
AssayDNA SNV/indelRNA fusionsMET ex14
LoD₉₅<3% VAF<100 copies<200 copies
Specificity100%100%100%
For reference, gold-standard NGS: tissue 2–5% VAF · blood 0.3–0.8% VAF (high input) rising to ~1.5–2.5% at low input. LiquiSmart matches or exceeds this — including from small samples. And in ONCO X validation, 96% of NGS-fail samples were rescued · 97%/100% PPA/NPA vs NGS.
100%specificity — tissue & blood
1%tissue failure rate — vs ~25% norm
96%of NGS-fail samples rescued
34hrsactionable report — even from a QNS sample
Patient
L.G.* · 71 years
Diagnosis
Stage IV NSCLC
Sample
Pleural biopsy — 10–15% tumor content
Prior testing
NGS & sequential PCR returned QNS

*Anonymised patient case.

Report in 34 hours.

LiquiSmart returned an actionable EGFR L858R mutation from the same low-content sample — enabling targeted therapy to begin immediately.

Patient case

When the sample is small, the result still has to count.

L.G. presented with a large pleural effusion and pleural nodularity. Pleural fluid was aspirated, but the cell block had insufficient cellularity for molecular studies. A pleural biopsy confirmed NSCLC — but at 10–15% tumour content it was deemed quantity not sufficient for NGS or sequential PCR.

With no molecular result and limited options, L.G. was started on chemotherapy — which he did not tolerate well. LiquiSmart returned the driver in 34 hours from the same sample.

Launched in India

Extensively validated. Available now.

  • Reagents developed under ISO 13485
  • Assay clinically validated at CLIA-certified, CAP-accredited laboratory
  • Independently validated by ONCO X for India · NABL accredited laboratory
  • Consistent performance across MD Anderson, UPenn, MCW & Fred Hutch
  • 77 clinical samples analysed across sites — pleural effusions, FNAs, FNA washes
  • Setup and training in 3 days

Order LiquiSmart Lung.

Request kits, connect to our scientific team, or explore a lab partnership.

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